Figure 4.

Phylogenetic tree of the angiosperm ABC1K proteins. The unrooted tree was based on the amino acid sequence alignment of the 126 ABC1K proteins from three eudicot species (Arabidopsis, M. truncatula, and P. trichocarpa) and four monocot species (Z. mays, O. sativa, B. distachyon, S. bicolor) which was manually corrected in the case of the truncated protein products. The tree was generated as outlined in figure 3. The three clades identified and illustrated in figure 3 are indicated here using the same color code and each of the 15 subfamilies are labeled. Subcellular localization of each subfamily (black – plastids, red – mitochondria) has been determined by experimental evidence or, in the absence of experimental evidence, by TargetP prediction. Bootstrap values are indicated.