Figure 1.

The effect of collagen (10 μg/mL) (Chrono-log Corporation) stimulation on CPCR in reconstituted whole blood and fresh whole blood.

A matrix system (Table I) was set up to provide nine variations of reconstituted whole blood (varying storage age of red cells and platelets), whilst the fresh whole blood was stored for a maximum of 4 hours. The figure shows the results for platelets stored for 1 day (A), 3 days (B), and 5 days (C). The samples were stimulated for a minimum of 5 minutes prior to analysis. All groups show a decrease in CPCR after stimulation with collagen. A significant decrease in platelet response to agonist was observed in control samples compared with stimulated samples (p<0.05, Wilcoxon’s test for paired samples). The results are presented as CPCR of control samples and stimulated samples. A statistically significant decrease of CPCR was found between fresh whole blood and all groups of reconstituted whole blood (p<0.05, Wilcoxon’s test for paired samples). P-values are indicated above the graphs.