The determination of CPCR in reconstituted whole blood and fresh whole blood after collagen (10 μg/mL) (Chrono-log Corporation) stimulation.
The figure shows the results for red blood cells stored for 0–4 days (A), 12–16 days (B), and 26–35 days (C). The samples were stimulated for a minimum of 5 minutes prior to analysis. All groups show a decrease in CPCR after stimulation with collagen. A significant decrease in platelet response to agonist was observed in control samples compared with stimulated samples (p<0.05, Wilcoxon’s test for paired samples). The results are presented as CPCR for control samples and stimulated samples. A statistically significant decrease of CPCR was found between whole blood and all groups of reconstituted whole blood (p<0.05, Wilcoxon’s test for paired samples). P-values are indicated above the graphs.