Figure 4.

One-pot PEGylation via SPAAC and fluorescent labeling via SML of TM₄₅₆AL: (A) SDS-PAGE Coomassie blue staining, (B) western blot analysis using anti-human TM mAb, (C) fluorescent image (Ex/Em = 488/526 nm), and (D) western blot analysis using anti-PEG mAb E11 of. The reactions of TM₄₅₆AL (10 µM), SrtA (5 µM), Gly₂-Bodipy (400 µM) and DBCO-PEG₅₀₀₀-OMe were taken place in 20 mM Tris (pH 8.0), 150 mM NaCl and 5 mM CaCl₂ at 37°C for 3 h.