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L-tyrosine levels correlate with melanin synthesis in P-MDCK cells. P-MDCK cells were grown in DMEM medium containing varying concentrations of L-tyrosine. MDCK cells were grown in DMEM medium containing 2 mM L-tyrosine. Melanin content was measured using a melanin solubilization assay (Materials and Methods) and normalized to protein content. Data are expressed as mean ± SD for n = 4.
