Figure 2. ALK and its ligands PTN and MDK are expressed in BCC tissue.
(A) mRNA expression of ALK was examined in total RNA extracted from bulk tissues across various skin conditions. SCC = squamous cell carcinoma. Error bars represent S.E.M. * indicates p<0.05 (one-way ANOVA). (B-D) ALK expression was examined at the protein level using a phosphorylated ALK antibody in (B) BCC, (C) normal skin, and (D) SCC. (E-F) MDK expression was examined at the protein level using a monoclonal antibody directed to MDK in (E) BCC and (F) normal skin. (G-H) PTN expression was examined at a protein level using a monoclonal antibody directed to PTN in (G) BCC and (H) normal skin. Scale bar = 100μm. (i) Double IF showing co-expression of PTN (red) and CD68 (green). Merged cells are in yellow.
