Figure 2. RNTS binds Bcr-Abl directly.

(A) F3NF, H2FA and H2FA™ are expressed in E.coli and purified with Ni⁺-NTA. Purified proteins and AP21967 consist of RNTS extracellularly. Bcr-Abl protein lysates were collected from K562 and K562/G01 cells. Pull-down assay was performed to detect the interaction between RNTS and Bcr-Abl in vitro. (B) K562 and K562/G01 cells were infected by Ad-F3NF and Ad-H2FA (or Ad-H2FA™), and then AP21967 was added. Co-immunoprecipitation assay was used to detect the interaction between RNTS and Bcr-Abl intracellularly.