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. 2013 Oct 28;4(12):2317–2325. doi: 10.18632/oncotarget.1464

Figure 3. The effect of HOXA11 on cell migration and proliferation.

Figure 3

(A) To analyze the effect of HOXA11 on cell migration and proliferation, H23 lung cancer cells were transfected with pAcGFP-HOXA11 fusion construct, or empty vector. After transfection, the expression of HOXA11 was confirmed by western blot using primary antibody directed to GFP (sc-9996; Santa Cruz Biotechnology, CA, USA). (B) H23 cells transfected with pAcGFP-HOXA11 were re-seeded in 6.5 mm Transwell® with 8 μm pore size (Corning, USA). The cells were induced to migrate into 1% of FBS containing media in 24 well plate. After 48 hr each transwell insert was stained by 1% crystal violet and destained with 10% acetic acid. The absorbance was measured at 564 nm using VERSAmax microplate reader (Molecular devices). (C) H23 cells transfected with pAcGFP-HOXA11 were seeded in a 96 well plate, and cell proliferation activity was measured every 24hrs with MTT assay. Y axis indicates absorbance measured at 570 nm using VERSAmax microplate reader (Molecular devices), and the data are presented as mean ± standard error (SE) of eight experiments.