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. 2013 Nov 6;4(12):2339–2353. doi: 10.18632/oncotarget.1446

Figure 7. Mutant MTSS1 inhibits PC3 and MDA-MB-231 cancer cells migration.

Figure 7

(A-D) PC3 (A) or MDA-MB-231 (C) cells were infected with pBabe-EV, pBabe-HA-wild-type-MTSS1 or pBabe-HA-S322A-MTSS1 retroviral vectors and after 3 days of puromycin (1 μg/ml) selection to eliminate the non-infected cells, the generated various cell lines were subjected to trans-well cell migration assay and photographed. Quantitative measurement of migrated PC3 (B) or MDA-MB-231 (D) cells was assessed after 12 hours. The number of cells was normalized against the number of cells in the corresponding pBabe-EV cells. The error bars represent mean ± SD (n= 3) * p< 0.05 (n= 3). (E-F) Scratch assays were performed with PC3 cells that were infected with pBabe-EV, pBabe-HA-wild-type-MTSS1 or pBabe-HA-S322A-MTSS1 retroviral vectors followed by 3 days of puromycin (1 μg/ml) selection to eliminate the non-infected cells. The generated various PC3 cell lines were seeded on a 6 well plate and scratched on the surface with a 200 μl pipette tip. Relative values were set at 1 of the gap width at the time of the scratch. Representative photographs at time points 0 and 18 hours after the scratch (E). Measurements were done in duplicate in 3 separate experiments, and data were depicted as average gap width (F). The error bars represent mean ± SD (n= 3). ***p<0.001; *p<0.05. (G-H) Scratch assays were performed with MDA-MB-231 cells that were infected with pBabe-EV, pBabe-HA-wild-type-MTSS1 or pBabe-HA-S322A-MTSS1 retroviral vectors followed by 3 days of puromycin (1 μg/ml) selection to eliminate the non-infected cells. The generated various MDA-MB-231 cell lines were seeded on a 6 well plate and scratched on the surface with a 200 μl pipette tip. Relative values were set at 1 of the gap width at the time of the scratch. Representative photographs at time points 0 and 18 hours after the scratch (G). Measurements were done in duplicate in 3 separate experiments, and data were depicted as average gap width (H). The error bars represent mean ± SD (n= 3). ***p<0.001; *p<0.05.