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. 2013 Nov 16;4(12):2366–2382. doi: 10.18632/oncotarget.1424

Figure 3. SR1 cells derived from various ovarian cancer cell lines show multiple differentiation potency.

Figure 3

(A, B) SR1, SR2, and their original counterpart cells from CP70 (A) and SKOV3 (B) cells were induced by adipogenesis-inducing medium for 14 days and stained with oil red O solution. Only SR1 and SR2 cells, but not the original cells, displayed lipid droplets in the cell body (white arrows). (C) Two independent clones of SR1 cells derived from OVCAR3 cells were seeded directly onto collagen-coating culture dishes in either adipogenesis-inducing medium or SR culture medium. Oil droplets were observed only in SR1 cells cultured in adipogenesis-inducing medium. (D) The osteogenesis capacity of SKOV3-derived SR1 cells and its original counterpart cells were examined. Only SR1 cells cultured in osteogenesis-inducing medium displayed osteocyte morphology with the osteogenic marker, ALP activity, 14 days after induction. Differentiated CP70SR1 were induced by osteogenesis (E) and chondrogenesis (F) and detected by specific staining. Undifferentiated CP70SR1 without induction retained a tumor-like morphology and showed no signals.