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. 2013 Nov 19;4(12):2397–2406. doi: 10.18632/oncotarget.1494

Figure 4. 1,25-VD promotes BCG induced THP-1 differentation/maturation.

Figure 4

(A) THP-1 cells were treated with vehicle, 1.25-VD, BCG, or combination overnight. Cells were collected for total RNA extraction. The gene expression levels of macrophage markers, CD163, CHIT1, and CH68 were determined by real-time PCR assay. (B) Representative photos display a morphological alteration after treatments were shown. (C) The expression of IL-8 after the treatment was detected by the real-time PCR and ELISA assays.