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(A) Immunoprecipitation assay (left pannel) demonstrates direct physical binding of EGFR and STAT3 induced by gefitinib treatment. Cells were treated with 8μM gefitinib for 6 hours. The samples were precipitated with STAT3 antibody and detected with antibodies against EGFR and STAT3 sequentially. (B) Immunoblotting analysis shows the effect of gefitinib on SOCS1 and SOCS3 in A549 cells.
