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A, Western blot analysis of the protein levels of the stem cell-associated transcription factors OCT4, NANOG, KLF4 and BMI1 in the ALDHhigh and ALDHlow subpopulations from C33A and HT-3 cells. GAPDH was used as a loading control. B, The expression of stem cell-associated transcription factors in tumorspheres formed by ALDHhigh and ALDHlow SiHa cells was measured by IHC analysis.
