Fig 5. NPY stimulates proliferation and migration of hypoxic ALDHhigh ES CSCs via the Y2R/Y5R pathway.
A. ALDHhigh and ALDHlow cells were pre-incubated for 6h in normoxia or hypoxia and then treated under the same conditions with the desired NPY receptor agonists (10−7M) or antagonists (10−6M) for 12h. Cells were subsequently pulsed with 10μM EdU, and proliferation was measured as a percent of EdU positive cells. B. ALDHhigh and ALDHlow cells were plated into BD FluoroBlok plates, treated as above for 18h and stained with Calcein AM. Cell migration was assessed based on fluorescence measured from the bottom of the plate.