Figure 1. Aberrant expression of IKKα and IKKβ proteins in HNSCC tumor specimens and cell lines.
A, Immunohistochemical analysis of IKKα, IKKβ, and phospho-IKK in representative tumor specimens and matched mucosa. H&E, pan-cytokeratin and rabbit IgG isotype stains served as controls. Magnification: 100X and 400X. B, Intensities of immunohistochemical staining of nuclear and cytoplasmic IKKs were semi-quantified and presented as histoscores of 18 HNSCC specimens. C, Overexpression of IKKα, IKKβ and phospho-IKK proteins in whole cell and nuclear lysates of 9 UM-SCC lines were compared with normal human primary keratinocytes (HeKa). β-actin and Ponceau-S staining as loading controls.
