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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Oncogene. 2013 Mar 4;33(9):1135–1147. doi: 10.1038/onc.2013.49

Figure 7. Knockdown of IKKs or AP1 subunits by siRNAs potently inhibit cell proliferation and migration.

Figure 7

A, IKKα and IKKβ double knockdown by siRNAs potently inhibited cell proliferation over single IKK knockdowns by MTT assay. Statistical significance of t-test, * single knockdown: p<0.05; +double knockdown: P<0.01. B, UM-SCC1 cells were transfected with IKK specific siRNAs for 48 hrs. Scratches were made on cell monolayers and wound closure was monitored at 14, 20, and 30 hrs. C, Quantification of wound healing with statistical significance. The distances between the wounds were measured and significant differences by individual IKK knockdowns were examined by t-test (*, p<0.05), or in dual IKK knockdown (+, p<0.01). D, Knockdown of FOSL and JunB individually by siRNAs inhibited cell proliferation by MTT assay. Statistical significance of t-test, * p<0.05. E, AP1 subunits were individually knocked down for 48 hrs. Scratches were made and wound closure was monitored at 11, and 15 hrs under serum free condition. F, Quantification of wound healing with statistical significance. The distances between the wounds were measured and significant differences were examined by t-test. *, p<0.05.