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. 2014 Feb 17;204(4):541–557. doi: 10.1083/jcb.201307050

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© 2014 Nazarko et al.

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Figure 3. — Atg37 is dispensable for the Cvt pathway and nonselective autophagy. (A) Atg37 is not required for the maturation of prApe1. Cells were grown in glucose medium (“Growth”) and then adapted to glucose medium without nitrogen for 6 h (“Starvation”). mApe1, mature Ape1; *, nonspecific band. (B) Atg37 is dispensable for cell survival during nitrogen starvation. Aliquots of cultures starved in glucose medium without nitrogen were plated on YPD medium. The number of cells able to form colonies was counted after 2–3 d of incubation. The data shown are from a single representative experiment out of two repeats. (C) Atg37 is not required for GFP-Atg8 processing by autophagy. Glucose-grown cells were adapted to glucose medium without nitrogen (SD-N).