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. 2014 Feb 17;204(4):541–557. doi: 10.1083/jcb.201307050

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© 2014 Nazarko et al.

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Figure 4. — Overexpression of Atg37 does not interfere with pexophagy. (A and B) Overexpression of Atg37 does not induce pexophagy (A) or affect the growth of cells in methanol medium (B). The data shown in B are from a single representative experiment out of two repeats. Ee, endogenous expression; Oe, overexpression. (C) Overexpression of neither N-terminal (GFP-Atg37) nor C-terminal (Atg37-GFP) fusions of Atg37 and GFP affects pexophagy. Methanol-grown cells were adapted to ethanol medium without nitrogen (SE-N). (D) Overexpression of Atg37 does not interfere with micropexophagy and macropexophagy. Cells were replica plated from methanol to glucose and ethanol plates, respectively. The residual activity of AOX was used to assess pexophagy.