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. 2014 Feb 17;204(4):541–557. doi: 10.1083/jcb.201307050

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© 2014 Nazarko et al.

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Figure 6. — Atg30 requires Atg37 for recruitment of Atg11 to the pexophagic RPC. (A) Atg37 is required for pexophagy induced by the overexpression of Atg30. (B) Atg37 is necessary for the growth suppression in methanol medium caused by the overexpression of Atg30. The data shown are from a single representative experiment out of two repeats. (C) Atg11 does not coimmunoprecipitate with Atg30 in the absence of Atg37. Methanol-grown cells were adapted to the glucose medium without nitrogen for 0.5 h. The results are presented for two independent colonies of Δatg37. *, nonspecific band. (D) Atg37 is required for the Atg30-dependent localization of Atg11 to the proximity of peroxisomes. Methanol-grown cells were adapted to the glucose medium without nitrogen for 1 h. Arrows point to the accumulation of GFP-Atg11 near the peroxisomes in WT cells and on the vacuolar membrane in the mutant cells. Bar, 5 µm.