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. 2014 Feb 17;204(4):497–505. doi: 10.1083/jcb.201309081

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© 2014 Veltman et al.

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Figure 1. — Folate chemotaxis is inefficient in axenic cells. Cells were grown under different conditions, then examined responding to linear attractant gradients in Insall chambers. Numbers are the means ± SEM of at least four independent experiments of at least 20 cells each. Tracks of cells from the same experiment have the same color. (A) Axenically cultivated AX2 cells responding to folate (left) and 4 h–starved cells responding to cAMP (right). (B) Bacterially grown NC4 cells (the parent of AX2) migrating toward folate. (C) Bacterially grown AX2 cells migrating toward folate.