Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 17;204(4):497–505. doi: 10.1083/jcb.201309081

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Veltman et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — PIP₃–labeled protrusions do not orient up folate gradients. (A) AX2 and NC4 cells transfected with the F-actin reporter Lifeact-mRFP and the PIP₃ reporter PH-CRAC-GFP were examined by confocal microscopy, and the presence or absence of a PIP₃ patch in each cell was determined during random migration. Bar, 10 µm. (B) Numbers of pseudopods with and without PIP₃ patches during random migration. Percentages are the means and standard deviations from three experiments. (C and D) Mean direction of protrusions during folate chemotaxis. (C) The displacements from the migration vectors of all pseudopods were measured, and the mean is shown in a circular plot. If all protrusions were extended in the same direction, a vector with length 1 would result. PIP₃-containing pseudopods from NC4 were too rare to give a reliable direction. (D) Rose plots of the directions of all protrusions with and without PIP₃. The shaded area indicates the 95% confidence interval of the mean angle. (E) Frequency and direction of pseudopods in C and D. (F) Rose plot of the direction of PIP₃ patches that form macropinosomes during folate chemotaxis.