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. 2014 Feb 17;204(4):507–522. doi: 10.1083/jcb.201310083

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© 2014 Gaggioli et al.

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Figure 6. — CDK regulates SLD-2 localization in the germline. (A) Confocal images of the distal region of the C. elegans gonad extruded from an sld-2::gfp, mcherry::histone h2b adult worm. This sld-2::gfp transgene is integrated as a single copy at a Mos transposon site and is expressed from the mex-5 promoter with a tbb-2 3′UTR. (B–D) As in A. The dotted line delineates the start of the transition zone as determined by the appearance of crescent-shaped nuclear histone signal. (E) As in A, but after cye-1 RNAi by feeding at 25°C. DIC, differential interference contrast. Bars, 10 µM.