Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 17;204(4):477–486. doi: 10.1083/jcb.201311014

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Richter et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Crystal structure of a MiD51 cytosolic domain sufficient for Drp1 recruitment. (A) MiD51-GFP and MiD51^ΔDR-GFP were expressed in wild-type MEFs and MEFs lacking endogenous MiD51 (MiD51^TALEN). Cells were subsequently immunostained for Drp1 and the mitochondrial marker protein Tom20 and visualized by fluorescence microscopy. (right) Linescans demonstrating colocalization of Drp1 with MiD51-GFP and MiD51^ΔDR-GFP. Bars, 20 µm. (B) Structure of MiD51^ΔN118 displaying the nucleotidyltransferase domain in blue and DRR domain in green. (C) Surface representation of MiD51^ΔN118; view and coloring as in B. (D) Topology of MiD51 and illustration of mutant constructs used in this study. TM, transmembrane domain; DR, disordered region; NT, nucleotidyltransferase domain.