Table 2.
ECM protein | Integrins | Cell source | Neural differentiation | Ref. |
Laminin Matrigel (rich in laminin) | α6β1 α3β1 | HESC lines: TE03 and TE06 | Neuronal generation and neurite outgrowth were significantly greater on laminin and laminin-rich Matrigel than fibronectin, poly-D-lysine, and collagen I | Ma et al[16], 2008 |
Fibronectin | α5β1 | Oligodendrocyte progenitor cells from rats | Promoted OPC survival, proliferation, migration, process extension, and OPC purity | Hu et al[35], 2009 |
Vitronectin | αvβ1, αvβ3, αvβ5 | hESC lines: Miz-hES4, Miz-hES6 | Promoted oligodendrocyte differentiation in the presence of RA/Shh/Noggin | Gil et al[36], 2009 |
Collagen IV | α1β1, α10β1 | Rabbit neural progenitor cells | Conducive for both neuronal and glial cell differentiation | Raghavan et al[61], 2013 |
Collagen I | α2β1 | Rabbit neural progenitor cells | Conducive for both neuronal and glial cell differentiation | Raghavan et al[61], 2013 |
Vitronectin-derived synthetic peptide acrylate surface | αvβ5 | hESC line: H1 | Promoted oligodendrocyte progenitor differentiation; higher NG2 expression compared to Matrigel | Li et al[13], 2013 |
Heparan sulfate | Binding heparin | Rabbit neural progenitor cells | Addition of heparan sulfate to collagen mixtures improved neuronal differentiation | Raghavan et al[61], 2013 |
ECM: Extracellular matrix; hESC: Human embryonic stem cells; RA: Retinoic acid; Shh: Sonic hedgehog; OPC: Oligodendrocyte progenitor cells.