Figure 1.

Time lapse analysis reveals DivIVA-GFP dynamics in B. subtilis. Cells expressing DivIVA-GFP under its native promoter were grown on agarose slides supplemented with LB at 37°C and analyzed microscopically. Pictures were taken every 2 min. After division DivIVA-GFP is mostly located at midcell or at one pole (white arrow). After 14 min DivIVA-GFP is recruited to new forming septa (red and blue arrow) and fluorescence intensity at the old septa decreases stepwise. For plotting the fluorescence measured at the old and new formed septa ROIs of identical size were drawn and the mean fluorescence of every spot was calculated individually.