Figure 4.

DivIVA-Dendra2 is dynamically recruited from the cell pole to the septa. DivIVA-Dendra2 fluorescence (green and red) was imaged before photoconversion using DIC, FITC, or TRITC specific filters. After photoconversion using a 405 nm laser (cyan circles) only red fluorescence (TRITC) and DIC was monitored to prevent additional photoconversion. After 5 min DivIVA-Dendra2 is recruited from the place of photoconversion (black and blue circle) to new septa forming (red arrow). A cartoon of the photoconversion is shown. The relative fluorescence of the left pole (black circle), the right pole (blue circle), and the new formed septa (red arrow) was measured. The relative fluorescence of the corresponding spots were calculated (CTF was calculated and the highest CTF of each spot was set as 1) and plotted. For every time point spots were chosen individually. Heat maps and corresponding histograms are shown below.