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. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: Cell Rep. 2013 Sep 26;5(1):151–165. doi: 10.1016/j.celrep.2013.08.026

Figure 1.

Figure 1

Splicing of S. cerevisiae pre-mRNAs studied in ensemble experimentsin vitro. (A) Spliceosome assembly and splicing pathway for RP51A pre-mRNA (Hoskins et al., 2011). Rectangles, exons; line, intron.(B)Cartoon showing the first and second chemical steps of splicing. (C) Products of the first and second step reactions of radioactively-labeled RP51A pre-mRNAincubated with WCE in the presence of ATPfor 0 – 60 min and visualized by denaturing PAGE. (D)Fraction of molecules that completed the first and second steps of splicing for eight different pre-mRNAs as a function of incubation time. (E) Compiled first (darker bars) and second step (lighter bars) rates (±S.E.) for the specified pre-mRNAs.