Table 1.

Primers used in the multilocus sequence typing, nature of genetic diversity, and expected sizes of the PCR products

Locus	Primers	Sequences (5' to 3')	Fragment sites(bp)a	Annealing Temperat-ure(°C)	References
18S rRNA	AL1687	TTC TAG AGC TAA TAC ATG CG	156-175	55	Xiao et al.,1999
	AL1691	CCC TAA TCC TTC GAA ACA GGA	1455-1475
	AL3032	GGA AGG GTT GTA TTT ATT AGA TAA AG	193-218	55
	AL1598	AAG GAG TAA GGA ACA ACC TCC A	1008-1029
COWP	Cry- 15	GTA GAT AAT GGA AGA GAT TGT G	921-943	52	Spano et al., 1997
	Cry-9	GGA CTG AAA TAC AGG CAT TAT CTT G	1445-1470
GP60	LX001 F1	ATA GTC TCG CTG TAT TC	4-21	50	Zhou et al., 2003
	LX002 R1	GCA GAG GAA CCA GCA TC	906-922
	LX003 F2	TCC GCT GTA TTC TCA GCC	9-27	50
	LX004 R2	GAG ATA TAT CTT GGT GCG	480-497
HSP70	F1	ACT CTA TGA AGG TAT TGA TT	922-941	55	Sulaiman et al., 2001
	R1	TTA GTC GAC CTC TTC AAC AGT TGG	2074-2051
	F2	CAG TTG CCA TCA GTA GAG	945-962	50
	R2	CAA CAG TTG GAC CAT TAG ATC C	2060-2039
	INT	GGA CGA GTT TGA ACA TCA A	1831-1849

^a Expected PCR product size for both Cryptosporidium hominis and C. parvum