Table 1.

Calculated IC₅₀ values from graphs in Figure 3 (μM)^a.

Kinase	Compound
	3	4	5	6	7
AKT1 (PKB alpha)	0.78	0.02	2.6	>100	3.6
AKT-2	0.72	0.02	1.9	>100	3.3
IKBKB (IKKβ)	72	1.1	4	15	34
RPS6K1	20	1.4	6	>100	12
AMPK (A1/B1/G1)	46	1.5	2.5	10	26
RAF1a	24	1.6	6.5	>100	8.5
MEK1a	12.8	1.1	9	>100	30
ERK2b	13	4	27	>100	20
NEK1	77	0.5	3.5	83	14
KDR (VEGFR2)	0.77	0.66	1.3	>100	6.5
MAPK14 (p38α)	92	35	>100	>100	>100
MAPKAPK2	>100	6.7	9.8	>100	16

^aAssays for RAF1 and MEK used the kinase cascade method diagramed in Figure 5 and discussed in the Materials and Methods section.

^bThe IC₅₀ values for ERK2 resulted from large experimental variability and should be regarded as only semiquantitative.