Table 1.

Cell recovery and yield of RNA from PS and SCLB purification methods.

Samples	PureSperm (PS)				Somatic Cell Lysis Buffer (SCLB)
	Input cells	Recovered cells	Recovery rate	RNA (ng) / 106 cells	Input cells	Recovered cells	Recovery rate	RNA (ng) / 106 cells
${\mathbf{S}}_{\mathbf{1}}^{\mathbf{P}}$	166.5	78	46.8%	37	166.5	132	79.3%	14
${\mathbf{S}}_{\mathbf{2}}^{\mathbf{P}}$	145	96	66.2%	47	145	108	74.5%	12
${\mathbf{S}}_{\mathbf{3}}^{\mathbf{P}}$	132.5	78	58.9%	86	132.5	120	90.7%	14
${\mathbf{S}}_{\mathbf{4}}^{\mathbf{P}}$	155	66	42.6%	23	155	132	85.2%	19
${\mathbf{S}}_{\mathbf{1}}^{\mathbf{L}}$	150	98	65.3%	29	150	120	80.0%	20
${\mathbf{S}}_{\mathbf{2}}^{\mathbf{L}}$	80	56	70.0%	26	80	94*	100.0%	38
${\mathbf{S}}_{\mathbf{3}}^{\mathbf{L}}$	150	84	56.0%	89	150	132	88.0%	30
${\mathbf{S}}_{\mathbf{4}}^{\mathbf{L}}$	70	70	100.0%	76	70	70	100.0%	15

The values in columns ‘input cells’ and ‘recovered cells’ are as million (× 10⁶). The samples from four subjects were denoted S₁, S₂, S₃, and S₄. P: pelleted storage; L: liquefied storage; Input cells: the number of sperm cells as initial input for purification; Recovered cells: the number of recovered sperm after purification; Recovery rate: the percentage of recovery; RNA (ng) / 10⁶ cells: the yield of isolated RNA (ng) from one million sperm cells;

^*: in one SCLB sample, the number of recovered cells is higher than the number of input cells. Probably this is related with sperm cell counting method. Its recovery rate is reset back to 100% in the table.