Table 2.
Reactivity of bulk CD8+ tumor infiltrating lymphocytes to autologous cancer cell lines
| Pt. 3 Gastric Ca. CD8+ TIL | Pt. 5 Biliary tract Ca. CD8+ TIL | Pt. 9 Colon Ca. CD8+ TIL | |||||||
|---|---|---|---|---|---|---|---|---|---|
| pg/ml IFN-γ | (% 4-1BB+) | pg/ml IFN-γ | (% 4-1BB+) | pg/ml IFN-γ | (% 4-1BB+) | ||||
|
|
|
|
|
||||||
| pOKT3 | 2302 | (92.3) | 1650 | (38.3) | 607 | - | |||
| Medium | 80 | (0.2) | 71 | (0.3) | 75 | (0.2) | |||
|
|
|
|
|
||||||
| Autologous cancer cell | GALMa | 1236 | (66.2) | BAOMa | 76 | (0.7) | CALM | 200 | (15.7) |
| GALMb | 50 | (1.7) | BAAM | 68 | (1.0) | ||||
| GALMc | 55 | (1.0) | |||||||
| GALMd | 2046 | (83.5) | |||||||
|
|
|
|
|
||||||
| Allogeneic cancer cell | Mel.624 | 37 | (0.3) | GALMa | 79 | (0.1) | GALMd | 62 | - |
| BAOMa | 66 | - | |||||||
NOTE: Mean IFN-γ secretion (pg/ml) in 24 h co-culture supernatants of CD8+ TIL with autologous and allogeneic cancer cell lines in representative independant experiments. After sampling each well for IFN-γ, TIL duplicates were pooled and harvested for 4-1BB staining for flow cytometry analysis (in parenthesis, percent of CD8+ cells, gated on CD3+ cells). Bolded values for IFN-γ reflect secretion of at least 200 pg/ml, whereas bolded 4-1BB+ reflects specific upregulation at least twice as high as the unstimulated condition (media).
Abbreviations: Pt., patient; pOKT3, plate bound anti-CD3.