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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biochem J. 2013 Sep 1;454(2):249–257. doi: 10.1042/BJ20130414

Figure 3. Sirt3 KO cells exhibited increased autophagy in response to starvation.

Figure 3

(A). Sirt3 WT and KO MEF cells were starved in HBSS for 0 and 4 hr. Western blot analyses were performed with anti- p-mTOR, total mTOR, p-p70S6K, and total p70S6K antibodies. β-actin western blots were used as loading controls. Quantification of p-mTOR (B), total mTOR (C), p-p70S6K (D), and total p70S6K (E) levels from western blot band intensity were shown in the graphs. *p<0.05 compared to 0 hr. (F). Sirt3 WT and KO MEF cells were starved in HBSS for 0 and 4 hr. Western blot analyses were performed with anti-LC3 antibody. β-actin western blots were used as loading controls. Quantification of relative LC3-I (G) and LC3-II (H) levels from western blot band intensity and shown in the graphs. *p <0.05 between WT and KO, †p<0.05 compared to 0 hr.