Fig. 2. Myeloid OX40L mRNA is upregulated by DEP-treated HBEC.
Immature mDC were cultured (48h) with pHBEC (2A), 16HBEC (2B) or alone (2C) in the absence or presence of DEP (3 μg/cm2), carbon (3 μg/cm2), MF1 or MF2 and for mDC alone, recombinant TSLP. Myeloid DC were isolated and OX40L mRNA measured by RT-PCR. Data are expressed as relative mRNA expression of OX40L compared to GAPDH (2ΔCt) (mean ± SE, n = 3, * = p < 0.05).