Immature mDC were cultured (48h) with DEP, Hunter College, or South Bronx fine ambient PM (3 μg/cm2), in the presence of untreated or treated 16HBEC and mRNA for OX40L (6A), Jagged-1 (6B), or Dll4 (6C) measured by RT-PCR. Data are presented as a representative experiment performed in triplicate and expressed as relative mRNA expression of OX40L, Jagged-1 or Dll4 compared to GAPDH (2ΔCt) (mean ± SE, in triplicate, * = p < 0.05).