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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Kidney Int. 2013 Jan 9;83(3):414–425. doi: 10.1038/ki.2012.426

Figure 3. Stable overexpression of c-mip in podocytes induces phenotypic and biochemical alterations.

Figure 3

(a) Confocal microscopy analysis of phalloidin staining in stable transfectant murine podocytes without [Tet(-)] and after induction of c-mip by tetracycline [Tet(+)]. c-mip–overexpressing podocytes display a loss of stress fibers, whereas the actin network is well preserved in non-induced stable transfectant cells. Scale bars, 20 μM. (b) Western-blotting of protein lysates from non-induced [Tet(-)] and induced [Tet(+)] c-mip stable transfectants. Overexpression of c-mip induces a downregulation of synaptopodin. (c) Downregulation of synaptopodin in membranous nephropathy (MN) and transgenic mice (Tg). Confocal microscopy analysis of synaptopodin on control human kidney (Con), kidney biopsy specimens from patients with MN, wild type (wt) and c-mip Tg mice. Scale bars, 20 μM.