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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Kidney Int. 2013 Jan 9;83(3):414–425. doi: 10.1038/ki.2012.426

Figure 6. Cyclosporine therapy inhibits c-mip expression and reduces proteinuria in PHN rats.

Figure 6

(a) Course of proteinuria during CsA therapy started from the day 21 post-first immunization. left, Urine samples (5 μl) and BSA (10 μg) were resolved by SDS-PAGE and gels stained with Coomassie Blue; right, course of proteinuria as assessed by calculating the proteinuria/urine creatinine ratio (UPr/UCr). (b) Quantitative RT-PCR of laser microdissected glomeruli from untreated [CsA(-)] and CsA-treated [CsA(+)] PHN rats (n=5 rats in each group). Relative copy numbers were calculated as described in Material and Methods; ** P <0.01, Mann-Whitney test. (c) Representative immunohistochemical analysis for c-mip in serial kidney sections from CsA(-) and CsA(+) PHN rats (day 42 post-immunization). Scale bars, 20 μm. (d-f) Western blotting of RhoA, ILK and DAPK on glomerular lysates from CsA(-) and CsA(+) PHN and control rats (Con), respectively.