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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Kidney Int. 2013 Jan 9;83(3):414–425. doi: 10.1038/ki.2012.426

Table 1.

Sequence of primers and PCR conditions.

Primers Sequence Accession number Expected size Ann Temp (°C) PCR cycles
Rat c-mip
Forward: TGTGTGCCTGGCTGCCATATATTCCTGCTATG NM_001163273.1 176 64 32
Reverse: GACAATGTGGCTTCCTGAGACACCAGGTC
18S
Forward: GTAACCCGTTGAACCCCATT NR_003278 151 60 16
Reverse: CCATCCAATCGGTAGTAGCG
Human c-mip Forward: CGTGTGCCTGGCTGCCATCTACTCCTGCTATG NM_198390 176 68 32
Reverse: GACAGCGTGGCTTCCTGAGACACCAGGTC
Human DAPK Forward: CTCAGGCGCATTGCTCAGCAGCTC 248
Reverse: GATGTCCATGGCATCGAGGATCTGC NM_004938
Human ILK Forward: GCAACGCAGTCGCCGTTCGCCT
Forward: CACGGTGTCCATGACTGGCTGCCA NM_001014795 210 58 35