TABLE 1.

Time course of macrophage cholesterol accumulation

Condition	Cholesterol			Esterified Cholesterol
	Total	Unesterified	Esterified
	nmol/mg cell protein			%
No addition (one day)	136 ± 16	136 ± 16	1 ± 0	1 ± 0
AcLDL (one day)	562 ± 23	461 ± 17	101 ± 6	18 ± 0
No addition (two days)	104 ± 2	104 ± 2	1 ± 2	1 ± 2
AcLDL (two days)	860 ± 75	488 ± 27	371 ± 51	43 ± 2

One-week-old human M-CSF-differentiated, monocyte-derived macrophage cultures were incubated for one or two days without or with 50 µg/ml AcLDL. Then macrophage cultures were analyzed for their unesterified and esterified cholesterol contents.