Fig. 5.

Cer generation results in the formation of gel-like domains. Mean fluorescence lifetime of t-PnA in mock- (A), CerS2- (B), and CerS5-transfected (C) HEK cells in the absence (•) and presence (○) of 0.5 U/ml of bSMase. D: Long lifetime component of t-PnA fluorescence intensity decay was measured in the absence (closed symbols) and in the presence (open symbols) of 0.5 U/ml of bSMase in mock- (circles), CerS2- (squares), and CerS5-transfected (triangles) cells. The values are the average (±SD) of n ≥ 3; all measurements were performed at 37°C.