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Locations of nested PCR primers that specifically amplify a portion of the fish CENP-A gene and not the histone H3 gene. Arrowheads indicate the 3′ ends anchored in codons that differentiate CENP-A from H3. CENP-A sequences: Dr: Danio rerio, Fr: Takifugu rubripes, Tn: Tetraodon nigrovirdis. Vertebrate histone H3: GgH3: Gallus gallus histone H3. Alignment based on Regnier et al. [15].
