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Nested primer pairs used to amplify Tallapoosa darter CENP-A gene from within exon 2 to within exon 4.
| Primer Name | Primer Sequence (5′ to 3′) | Annealing Temperature (°C) |
|---|---|---|
| S2L | TCGNCCNGGRACNARGGCCCTRATG | 66 |
| A5L | TGGCCARCTGNANGTCRCGNGGRAA | 66 |
|
| ||
| S3s | GGCCCTRATGGARATYCGCAA | 64 |
| A3s | ATGGATGGCACANARRTTNGC | 64 |
