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Nested primers for PCR based walking reactions.
| Primer Name | Primer Sequence (5′ to 3′) |
|---|---|
| F2(AP1) | CAGAAAACATGCCGAGTCTTACTCCTG |
| F1(AP2) | CTCCTCGTGGTTTGCTTCTGTTTGACT |
|
| |
| BC(AP1) | CAAGTTATAGAAGCTGCATTTATTGTTTTC |
| BB(AP2) | GTGCCTGGTGATGTTTAGTTTTGTAAC |
|
| |
| W2C(AP1) | TCTGACAGAAAGTGTTCGCTCCCAGAC |
| W2A(AP2) | CAGTAGGAGTCCCATGTGCAATAAATCGG |
