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Primers utilized for amplification of CENP-A gene from Percina species.
| Primer Name | Primer Sequence (5′ to 3′) | Annealing Temperature (°C) |
|---|---|---|
| EAFo | TCTCCGGGCTTCAAAACATGCTCCTC | 63 |
| tdA5L | TGGCCAGCTGAATGTCCCGAGGGAA | 63 |
|
| ||
| EAFi | GCGGATTCGTGCTCCCGTTCA | 63 |
| tdA3s | GTGGATCGCACACAGGTTCGC | 63 |
|
| ||
| fwPA1 | TGCGTGATGAATACAGTGCCTGGT | 63 |
| fwPR1 | CCGCGTCCCAAACACACCGA | 63 |
| revMTF6 | TCCCTGGTGGTCCAGTGTCTGTC | 63 |
