Table S1.
Characteristics of Hrc predicted proteins of P. savastanoi TTSS.
| Name | length (aa) | ave. hyd. | TM-Reg | sign | loc | Ccoil reg. | CDDs | Homology (%) | ||
|---|---|---|---|---|---|---|---|---|---|---|
| HrcC | Psf | 699 | −0,326609 | (S) | - | + (1–23) | OM | 307–334 | + | 99.0 |
| Psv | 699 | −0,326609 | (S) | - | + (1–23) | OM | 307–334 | + | 99.0 | |
| Psn | 699 | −0,319456 | (S) | - | + (1–23) | OM | 307–334 | + | 99.0 | |
| HrcJ | Psf | 268 | 0.216418 | (M) | 2 (P1–23 / P220–242) | - | IM | 26–58, 91–127 | + | 98.0 |
| Psv | 268 | 0.211567 | (M) | 2 (P1–23 / P220–242) | - | IM | 26–58, 91–127 | + | 99.0 | |
| Psn | 268 | 0.211567 | (M) | 2 (P1–23 / P220–242) | - | IM | 26–58, 91–127 | + | 99.0 | |
| HrcN | 449 | 0.003341 | (S) | - | - | C | 202–231, 358–406 | + | 99.0 | |
| HrcQa | 238 | −0.123950 | (S) | - | - | C | 121–157, 207–238 | + | 98.0 | |
| HrcQb | 129 | −0.571317 | (S) | - | - | C | - | + | 100.0 | |
| HrcR | Psf | 208 | 0.719712 | (M) | 4 (P8–30 / P44–66 / P150–172 / P179–201) | - | IM | 62–103 | + | 100.0 |
| Psv | 208 | 0.719712 | (M) | 4 (P8–30 / P44–66 / P150–172 / P179–201) | - | IM | 62–103 | + | 100.0 | |
| Psn | 208 | 0.710577 | (M) | 4 (P8–30 / P44–66 / P150–172 / P179–201) | - | IM | 62–103 | + | 99.0 | |
| HrcS | 88 | 1,061364 | (M) | 3 (P10–32 / S42–64 / S67–88) | - | IM | - | + | 100.0 | |
| HrcT | 264 | 0.936364 | (M) | 7 (S12–34 / S43–65 / P76–98 / S134–156 / P186–208 / P217–239 / P243–264) | + (1–37) | IM | - | + | 99.0 | |
| HrcU | 359 | 0.140111 | (M) | 5 (S26–48 / P73–95 / S132–154 / S157–179 / P183–205) | - | IM | 201–248 | + | 99.0 | |
| HrcV | 695 | 0.266475 | (M) | 6 (P31–53 / S71–93 / P108–130 / P203–225 / S237–259 / P296–318) | + (1–17) | IM | 473–563 | + | 99.0 |
Columns, ordered from left to right indicate: (1) Putative protein name; (2) Name of the corresponding pathovar when non synonymous mutations are present; (3) Length of putative protein; (4) Average of hydrophobicity, according to SOSUI [31]; (5) S = Soluble, M = Membrane, as predicted by SOSUI [31]; (6) Presence, number and type (P = Primary, S = Secondary) of Trans-Membrane regions, predicted by SOSUI [31]; (7) Presence of Signal Peptide, predicted by SOSUIsign [32]; (8) Localization of putative protein (EC = Extracellular, C = Cytoplasm, OM = Outer Membrane, P = Periplasm, IM = Inner membrane, U = Unknown), predicted by SOSUIgramN [33]; (9) Presence of Coiled-coil regions, predicted by SOSUIcoil [34]; (10) Presence of Conserved Domains, evaluated using BLAST-CDDs [35]; (11) Percentage of homology with the same predicted protein from P. syringae pv. phaseolicola 1448A, evaluated with ClustalW [36].