Table S2.
Characteristics of Hrp predicted proteins of P. savastanoi TTSS.
| Name | length (aa) | ave. hyd. | TM-Reg | sign | loc | Ccoil reg. | CDDs | Homology (%) | ||
|---|---|---|---|---|---|---|---|---|---|---|
| HrpA | 108 | −0.331482 | (S) | - | - | EC | 59–105 | + | 97.0 | |
| HrpB | 124 | −0.184678 | (S) | - | - | EC | 52–90 | + | 99.0 | |
| HrpD | 176 | −0.253977 | (S) | - | - | C | - | - | 97.0 | |
| HrpE | 193 | −0.180311 | (S) | - | - | C | 34–99 | + | 100.0 | |
| HrpF | 74 | −0.393243 | (S) | - | - | C | - | + | 100.0 | |
| HrpG | 146 | 0.042466 | (S) | - | - | C | - | - | 98.0 | |
| HrpJ | 368 | −0.305978 | (S) | - | + (1–30) | OM | 30–76, 82–157, 197–228, 245–278, 330–368 | + | 99 0 | |
| HrpL | Psf | 184 | −0.438587 | (S) | - | - | C | 152–179 | + | 98.0 |
| Psv | 184 | −0.43858 | (S) | - | - | C | 152–180 | + | 98.0 | |
| Psn | 184 | −0.460870 | (S) | - | - | C | 152–181 | + | 98.0 | |
| HrpO | 148 | −1.135810 | (S) | - | - | C | 10–148 | + | 99.0 | |
| HrpP | 190 | −0.461053 | (S) | - | - | P | 138–165 | + | 98.0 | |
| HrpQ | 324 | −0.169445 | (S) | - | - | C | 220–250 | + | 99.0 | |
| HrpR | 306 | −0.113072 | (S) | - | - | C | 261–304 | + | 99.0 | |
| HrpS | Psf | 302 | −0.106954 | (S) | - | - | C | 105–142, 246–286 | + | 99.0 |
| Psv | 302 | −0.098344 | (S) | - | - | C | 105–132, 246–286 | + | 99.0 | |
| Psn | 302 | −0.106954 | (S) | - | - | C | 105–142, 246–286 | + | 99.0 | |
| HrpT | 67 | −0.167164 | (M) | + (P1–23) | + (1–29) | U | - | - | 100.0 | |
| HrpV | Psf | 115 | −0.217391 | (S) | - | - | C | 40–73 | - | 99.0 |
| Psv | 115 | −0.217391 | (S) | - | - | C | 40–73 | - | 100.0 | |
| Psn | 115 | −0.205218 | (S) | - | - | C | 40–80 | - | 99.0 | |
| HrpZ | 347 | −0.275792 | (S) | - | + (1–23) | EC | 24–62 | + | 98.0 |
Columns, ordered from left to right indicate: (1) Putative protein name; (2) Name of the corresponding pathovar when non synonymous mutations are present; (3) Length of putative protein; (4) Average of hydrophobicity, according to SOSUI [31]; (5) S = Soluble, M = Membrane, as predicted by SOSUI [31]; (6) Presence, number and type (P = Primary, S = Secondary) of Trans-Membrane regions, predicted by SOSUI [31]; (7) Presence of Signal Peptide, predicted by SOSUIsign [32]; (8) Localization of putative protein (EC = Extracellular, C = Cytoplasm, OM = Outer Membrane, P = Periplasm, IM = Inner membrane, U = Unknown), predicted by SOSUIgramN [33]; (9) Presence of Coiled-coil regions, predicted by SOSUIcoil [34]; (10) Presence of Conserved Domains, evaluated using BLAST-CDDs [35]; (11) Percentage of homology with the same predicted protein from P. syringae pv. phaseolicola 1448A, evaluated with ClustalW [36].