Table S3.
Real-Time gene expression analysis for selected TTSS genes of P. savastanoi.
| Normalized expression (n-fold) ± sd * | ||||
|---|---|---|---|---|
|
| ||||
| Psv 5 | ||||
|
| ||||
| 3 h | 6 h | 18 h | 24 h | |
|
| ||||
| hrpA | 10.2 ± 1.4 | 9.9 ± 0.8 | 31.1 ± 4.1 | 124.7 ± 9.9 |
| hrpS | 14.8 ± 0.9 | 22.4 ± 2.0 | 37.3 ± 3.3 | 152.1 ± 11.1 |
| hrpL | 11.8 ± 0.9 | 15.8 ± 2.3 | 29.9 ± 2.0 | 134.7 ± 8.7 |
| hrpV | 4.4 ± 0.2 | 53.2 ± 6.3 | 19.5 ± 1.5 | 32.1 ± 3.4 |
|
| ||||
| Psn 23 | ||||
|
| ||||
| 3 h | 6 h | 18 h | 24 h | |
|
| ||||
| hrpA | 18.33 ± 2.1 | 21.34 ± 3.36 | 54.59 ± 6.43 | 23.97 ± 3.2 |
| hrpS | 41.7 ± 3.9 | 24.43 ± 2.9 | 50.65 ± 7.23 | 14.6 ± 2.2 |
| hrpL | 29.87 ± 3.11 | 4.99 ± 0.98 | 11.2 ± 1.4 | 22.4 ± 2.1 |
| hrpV | 78.99 ± 8.45 | 21.3 ± 3.43 | 7.78 ± 0.9 | 7.66 ± 1.1 |
|
| ||||
| Psf 134 | ||||
|
| ||||
| 3 h | 6 h | 18 h | 24 h | |
|
| ||||
| hrpA | 134.78 ± 10.9 | 90.23 ± 7.98 | 4.89 ± 1.1 | 0.98 ± 0.23 |
| hrpS | 31.21 ± 4.22 | 27.33 ± 4.3 | 5.23 ± 1.43 | 1.21 ± 0.4 |
| hrpL | 6.11 ± 1.98 | 40.23 ± 2.34 | 3.5 ± 0.12 | 1.11 ± 0.03 |
| hrpV | 7.68 ± 2.31 | 7.98 ± 2.1 | 5.4 ± 0.45 | 78.92 ± 6.15 |
*
Normalized fold increase in the expression of each analyzed gene in Psv5, Psn23 and Psf134 grown in MM vs. KB at 3, 6, 18 and 24 h after bacterial inoculation on fresh medium. Standard deviations (sd) are reported.