. 2011 Oct 28;2(4):788–803. doi: 10.3390/genes2040788

Table 2.

m/z values of intact flagellin and each peptide fragment ^*.

pv.	intact (A2-Q282) ^a			N136-R210 b			D200-A211 b

	calcd. [M+H] ⁺	Observed ^c	Δ	calcd. [M+H]⁺	Observed ^c	Δ	calcd. [M+H]⁺	Observed ^d	Δ
Pta	29,147	32,487	3,300	7,388	10,611	3,220	1,276	1,813	537
Pph	29,147	32,545	3,400	7,388	10,616	3,230	1,276	e
Pto	29,044	32,321	3,300	7,287	10,513	3,230	1,276	1,813	537
Psy	29,178	32,393	3,200	7,385	10,446	3,060	1,319	1,828	509
pv.	D139-F167 ^b			D168-A188 ^b			E189-I199 ^b

	calcd. [M+2H]²⁺	Observed ^d	Δ	calcd. [M+2H]²⁺	Observed ^d	Δ	calcd. [M+H]⁺	Observed ^d	Δ

Pta	1,440	1,977	537 × 2	954	1,491	537 × 2	1,107	1,644	537
Pph	1,440	^e		954	^e		1,107	^e
Pto	1,433	1,970	537 × 2	723	1,261	537 × 2	1,020	1,557	537
Psy	1,426	1,935	509 × 2	945	1,454	509 × 2	1,123	1,632	509

The m/z values of A2-Q282 and N136-R210 are described using averaged atomic weights. Those of D139-F167, D168-A188, E189-I199 and D200-A21 are expressed as monoisotopic ions;

There is no translation start codon (Met) in the mature flagellin;

The digestion of flagellin by trypsin gave the glycopeptide N136-R210, and by Asp-N gave four glycopeptides, D139-F167, D168-A188, E189-I199 and D200-A211;

Ions observed in the MALDI-TOF mass spectrum. The measurement errors for intact proteins and the trypsin-digested proteins are around 300 ppm and 100 ppm, respectively;

Ions observed in ESI mass spectra;

Not determined.