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. 2011 Aug 16;2(3):608–626. doi: 10.3390/genes2030608

Figure 6.

Figure 6

FE spectra of ANS with proteins: ubiquitin (solid circles), Ub38 mm (open circles), Ub71L (open squares), and Ub71h (solid triangles). Samples contained 10 μM protein and 250 μM ANS in 12.5 mM KH2PO4, 50 mM NaF, pH 7.5 containing either no GuHCl (a); or 2 M GuHCl (b). Excitation wavelength was 340 nm.