Figure 3.

Effect of nCUR versus sCUR on bacterial adherence.M. catarrhalis adherence assay. After pre-incubation of Detroit cells for 60 minutes with nCUR or sCUR, respectively, at the indicated concentrations, bacteria (M. catarrhalis ATCC 25238) were inoculated onto the monolayers at a MOI of 30, centrifuged, and incubated for 30 min at 37°C. Non-adherent bacteria were removed and adherent bacteria were determined by quantitative culture of trypsinized cells. The overall ANOVA test statistic revealed a p < 0.0001, the between-column values calculated using Bonferroni’s multiple comparisons test were >0.05 for nCUR versus sCUR at 100 or 200 μM, respectively. Mean adherence ratios + 1 SD of three independent experiments, each run in triplicate, are shown. MEM containing 0.1% DMSO, but no CUR, was used as control.