Figure 2.
Constitutive expression of the G5A fusion protein in all tissues of Arabidopsis thaliana seedlings. Seven (A–F) or 21 day-old (G–J) plants of the G5A line were checked for reporter expression by excitation of GFP (at λex = 488 nm) under a stereo microscope (A,B) or a confocal microscope (C–J). Fluorescence emission by GFP is shown in (A,C,E,G,I) and corresponding bright field images are shown respectively in (B,D,F,H,J). Fluorescent stereo microscope observation of intact seedlings allowed detection of GFP signals in cotyledons and the primary root (A,B). Confocal microscopy observation of leaf epidermal cells (C,D) and mesophyll cells (E,F) showed good expression of G5A in leaf tissues. G5A fluorescence was observed in both the cytosol and the nucleus (arrows) of root cells (G,H) and of stomatal guard cells (I,J). Scale bar = 20 μm.